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Journal of Intelligent Material Systems and Structures
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Genetically Engineered Calmodulin Self-Assembled on Gold Surface

Eiry Kobatake

Shigeya Suzuki

Yasuko Yanagida

Tetsuya Haruyama

Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

Masuo Aizawa

Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japanmaizawagbio.titech.ac.jp

In order to construct intelligent materials consisting of functional proteins, the assembling techniques of proteins with retaining their functions even on a solid surfaces are required. Calmodulin, which expresses modulating function by conformation change with calcium binding, was selected as a model protein for molecular assembling. Calmodulin was genetically engineered for self-assembling on the gold surface with an oriented manner by introducing cysteine residues at the N-terminus of calmodulin. The engineered calmodulin retained Ca2+-responsive modulating function of enzyme activity as native calmodulin. Enzyme immunoassay and quartz crystal microbalance revealed that the engineered calmodulin was efficiently assembled on the gold surface in an acidic buffer solution as compared with native calmodulin. Genetic engineering is discussed to be effective on modifying proteins to be self-assembled on solid matrices.

Journal of Intelligent Material Systems and Structures, Vol. 10, No. 6, 446-450 (1999)
DOI: 10.1106/YTOH-4UCN-C96V-N5TK
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